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用ＭＳＣａ培养基从甘蔗ＰＣ９１０２嫩叶诱导愈伤组织。接种３０～４０天后，将愈伤组织转入ＡＡ液体培养基，进行悬浮培养、经过５０～８０天，建立起典型胚性细胞悬浮培养物，分离到原生质体、原生质体用ＭＲＰ１－ＭＲＰ２琼脂糖培养基培养，１６～２０天后给予光照，２５～３０天后转入Ｎｅ分化培养基，４５天左右可见绿芽分化。重复试验表明，从外植体诱导愈伤组织开始，建立悬浮细胞系，分离原生质体到培养再生完整小植株的全过程约２００天。
Callus was induced from young leaves of sugarcane PC9102 using MSCa medium. After 30 to 40 days of inoculation, the callus was transferred to AA liquid medium and cultured in suspension. After 50 to 80 days, a typical embryogenic cell suspension culture was established and protoplasts were isolated. Protoplasts were stained with MRP1-MRP2 agarose After 16 to 20 days light irradiation was given, and 25 to 30 days later it was transferred to Ne differentiation medium. About 45 days, the differentiation of green shoots was observed. Repeated experiments showed that starting from the explant-induced callus, the establishment of a suspension cell line, protoplast isolation and regeneration of whole plants to regenerate the entire process of about 200 days.