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目的:探讨3月龄SD大鼠去卵巢短程和长程骨量的变化,以指导临床用药。方法:30只3月龄SD雌性大鼠,随机分为基础对照组、年龄对照(30天和90天)组、去卵巢(30天和90天)组,切除双侧卵巢建立骨质疏松症模型,实验结束,取胫骨近端行不脱钙骨制片进行骨组织形态计量学分析。结果:实验30天去卵巢大鼠与年龄对照,骨形成的参数值(荧光标记周长百分率、骨矿化沉积率、骨形成率)和骨吸收的参数值(破骨细胞数目)都明显增加。但骨吸收大于骨形成,造成骨量丢失(骨小梁面积百分率明显减少),骨结构变差(骨小梁数目减少,分离度增加),90天去卵巢大鼠组与对照组比较,荧光标记周长百分率减少,骨矿化沉积率增加,破骨细胞数目显著增加,骨吸收增强,骨质丢失,出现骨质疏松。结论:30天去卵巢大鼠呈骨高转化,但90天去卵巢大鼠表现为骨形成参数有下降趋势,同时骨吸收增加,骨量丢失,造成骨质疏松。
Objective: To investigate the changes of short-term and long-term bone mass in ovariectomized 3-month-old SD rats to guide clinical medication. Methods: Thirty female SD rats of 3 months old were randomly divided into basic control group, age control group (30 days and 90 days), ovariectomized (30 days and 90 days) group, ovariectomized and osteoporosis Model, the end of the experiment, take the proximal tibia non-decalcified bone preparation bone histomorphometry analysis. RESULTS: In the ovariectomized 30-day experimental group, the parameters of bone formation (the percentage of perimeter of fluorescent marker, the rate of bone mineralization, the rate of bone formation) and the parameter of bone resorption (the number of osteoclasts) were significantly increased . However, bone resorption was greater than bone formation, resulting in loss of bone mass (trabecular area percentage was significantly reduced), bone structure deterioration (trabecular number decreased, increased resolution), 90-day ovariectomized rats compared with the control group, fluorescence Marked perimeter percentage decreased bone mineral deposition increased, a significant increase in the number of osteoclasts, increased bone resorption, loss of bone, osteoporosis occurs. CONCLUSION: In the ovariectomized 30-day-old rats, the bone is highly transformed, but the parameters of bone formation in the 90-day ovariectomized rats show a decreasing trend. At the same time, the bone resorption increases and the bone mass is lost, resulting in osteoporosis.