论文部分内容阅读
为了在分子水平上探讨棉花叶绿素质量分数的遗传规律,利用重组近交系群体T586×渝棉1号的270个F2:7家系为材料,构建了包括604个标记和覆盖棉花基因组3 140.9 cM的重组近交系遗传连锁图谱;2007-2008年在西南大学歇马科研基地,用日本产叶绿素计(SPAD-502)测定亲本和家系的叶绿素质量分数;采用复合区间作图法(MQM),对棉花叶绿素质量分数进行数量性状位点(QTL)分析.检测到4个与叶绿素质量分数相关的QTL,分别位于第6,15,19和25染色体,其中有2个QTL在1个环境下检测到,解释表型变异的5.4%和4.6%;在2个环境下检测到2个QTL,解释表型变异的8.2%,4.8%,23.9%和14%.本研究的QTL定位将有助于叶绿素质量分数的精细定位和棉花高光效育种的分子标记辅助选择.
In order to explore the genetic rule of chlorophyll content in cotton, 270 F2: 7 lines of T586 × Yumian 1 were used as materials to construct a genetic map including 604 markers and 3 140.9 cM Recombinant inbred line genetic linkage map; from 2007 to 2008 in Southwest University Xie Ma scientific research base, with the Japanese production of chlorophyll meter (SPAD-502) to determine the chlorophyll content of parents and families; using composite interval mapping (MQM) Quantitative trait loci (QTLs) were used to analyze the chlorophyll content of cotton.The four QTLs related to chlorophyll content were detected on chromosomes 6, 15, 19 and 25, respectively, of which 2 were detected in 1 environment , Explaining 5.4% and 4.6% of the phenotypic variation, 2 QTLs detected in 2 environments, accounting for 8.2%, 4.8%, 23.9% and 14% of the phenotypic variation.The QTL mapping in this study would contribute to the chlorophyll Fine-grained mass fraction and molecular marker-assisted selection for high-efficiency cotton breeding.