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目的：研究抗角蛋白自身抗体（ＡＫａｕｔｏＡｂ）对白细胞介素１β（ＩＬ－１β）诱发角朊细胞增殖的影响．方法：采用角朊细胞无血清培养方法，以３Ｈ－ＴｄＲ标示角朊细胞增殖程度，检测ＩＬ－１β刺激角朊细胞及ＡＫａｕ－ｔｏＡｂ处理角朊细胞的增殖代谢．结果：１６００Ｕ／ｍｌ及以上浓度ＩＬ－１β对角朊细胞增殖有明显促进作用（Ｐ＜０．０１），正常血清浓度ＡＫａｕｔｏＡｂ对ＩＬ－１β引起的角朊细胞增殖有抑制作用（Ｐ＜０．０１）．结论：ＡＫａｕｔｏＡｂ抑制ＩＬ－１β引起的角朊细胞增殖，提示皮肤炎症反应中，角朊细胞内沉积的ＡＫａｕｔｏＡｂ对角朊细胞的过度增殖有调节作用．
Objective: To investigate the effect of anti-keratin autoantibodies (AKautoAb) on the proliferation of keratinocytes induced by interleukin-1β (IL-1β). Methods: Proliferation of keratinocytes was detected by 3H-TdR and the proliferation of keratinocytes stimulated by IL-1β and AKau-toAb were detected by serum-free culture of keratinocytes. Results: The proliferation of keratinocytes was significantly enhanced at a concentration of 1600 U / ml and above (P <0.01). The normal serum concentration of AKautoAb could inhibit the proliferation of keratinocytes induced by IL-1β (P <0.01). 01). CONCLUSION: AKautoAb inhibits the proliferation of keratinocytes induced by IL-1β, suggesting that AKautoAb deposited in keratinocytes may play a regulatory role in the hyperproliferation of keratinocytes in the skin inflammatory response.