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Paulson和Polakoski用一束包扎松驰的玻璃毛纤维取出人类精液中不活精子。这一操作可能引致精子超微结构的损伤和随着时间的延长精子活动力大幅度下降,但在过滤的精液中精子活动力的百分比即使经过12小时培养也比未过滤的精子活动力的百分比为高。包扎玻璃纤维必需均匀。太紧,则不管精子如何活跃和有力也不能穿透。但如纤维压力太松,精液中一些死精子和残屑就有可能穿透。一种可靠的和可资推广的方法是对排出的低质量精液中活精子进行分离和浓缩,这对人工授精和冷冻储藏都具有临床意义。
Paulson and Polakoski remove inactivated sperm in human semen with a bundle of loose glass wool fibers. This may result in damage to the ultrastructure of the sperm and a significant decrease in sperm motility over time, but the percentage of sperm motility in the filtered semen is even greater than the percentage of unfiltered sperm motility even after 12 hours of culture High. Bandaged fiberglass must be uniform. Too tight, no matter how active and powerful sperm can not penetrate. However, if the fiber pressure is too loose, some dead sperm and debris in the semen may penetrate. A reliable and widely available method for separating and concentrating live spermatozoa from discharged low-quality semen is clinically significant for both artificial insemination and cryogenic storage.