研究了CTAB—正辛醇—正庚烷反胶束溶液萃取牛血红蛋白(PH_b)时、pH值、表面活性剂浓度、助表面活性剂浓度、离子种类和离子强度、溶剂比以及蛋白质浓度等因素对萃取效果的影响,并以蛋白质分子与表面活性剂分子间的相互作用以及反胶束大小的空间阻碍作用上进行了解释.研究表明,水相PH值在10.5～12.5之间,KCI浓度为0.1mol/l,反胶束溶液中表面活性剂浓度为0.02mol/l,正辛醇与正庚烷之比为0.25,反胶束溶液与水溶液体积比为1.0时,血红蛋白的萃取率可达93%以上. The effects of pH value, surfactant concentration, cosurfactant concentration, ionic species and ionic strength, solvent ratio, and protein concentration on the extraction of bovine hemoglobin (PH_b) by CTAB-n-octanol- On the extraction effect, and the interaction between the protein molecules and surfactant molecules and the size of the reverse micelle space obstruction on the role of the study showed that the aqueous PH value of 10.5 to 12.5, KCI concentration of 0.1mol / l, reverse micelle solution surfactant concentration of 0.02mol / l, n-octanol and n-heptane ratio of 0.25, reverse micelles solution and aqueous solution volume ratio of 1.0, the hemoglobin extraction rate up to 93% or more.