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作者根据鹤草酚驱绦药理实验提示,行进了抗肿瘤方面应用探讨,所用制剂由药剂教研室新剂型小组提供(鹤草酚精氨酸盐注射液)。通过伊红染色法体外对肝癌细胞作用实验证明,当鹤草酚为2.5, 1.25, 0.63, 0.31, 0.16, 0.08mg/ml浓度时,作用1小时的赤染率分别为100.0, 100.0, 99.8, 94.5, 38.0, 10.0%,作用3小时的赤染率均为100%。对S_(37), U_(14), S_(180),肝癌肉瘤、肝癌腹水癌等五种瘤谱的接种动物治疗实验证明,腹腔给药30mg/kg鹤草酚对S_(37),肉瘤抑制率47.0%,与对照组比较差异显著( P<0.01),对U_(14)肉瘤抑制率38.7 (P<0.05),对S_(180)肉瘤和肝癌肉瘤抑制率分别为26.3%和23.5% (P>0.05),对{1 l}a}俊水癌洽药组与对照组小鼠存活天数分AIJ为26.2天和17.5.天,生命延fC率为49.6%。对S,8o和53S37两肉瘤,在药物总剂量与上相同情况下,用瘤体局部和腹腔同时给药,其肿瘤抑制率分别增至66.7%和65.3%,证明该药对上述五种瘤种均有抑制作用,并用局部给药效果提高。本文测定了该制剂小鼠腹腔给药的LDS。为90.7±4.9mg/kgo每天小鼠腹控给药一次,连续10天的L,D s o为67.7±4.lmg/kg。上四种肉瘤体内实验给药14天,洽药与对照组:;]]物体重(均去瘤体)增长速度基本一致,用此剂量没见明显毒性。
According to the pharmacological experiment of repelling geranium, the authors proceeded to discuss the application of anti-tumor drugs. The preparations used were provided by the new formulation group of the Pharmacy and Pharmaceutical Research Office (Medicinol arginine injection). Experiments on hepatocarcinoma cells in vitro by eosin staining showed that when the concentration of apigenin was 2.5, 1.25, 0.63, 0.31, 0.16, and 0.08 mg/ml, the red dye staining effect for 1 hour was 100.0, 100.0, 99.8, 94.5, respectively. , 38.0, 10.0%, the red dyeing rate was 100% for 3 hours. The treatment of S_ (37), U_ (14), S_ (180), hepatoma sarcoma, and ascites carcinoma of the liver inoculated with five tumors showed that the intraperitoneal administration of 30 mg/kg retinol against S_ (37), sarcoma The inhibition rate was 47.0%, which was significantly different from that of the control group (P<0.01), the inhibition rate of U 14 sarcoma was 38.7 (P<0.05), and the inhibition rates of S 180 sarcoma and hepatocellular carcinoma sarcoma were 26.3% and 23.5%, respectively. (P>0.05), the survival days of the {1 l}a} Jun Shui-Shui He-Xiao and control groups were 26.2 days and 17.5 days, and the survival rate was 49.6%. For S, 8o, and 53S37 sarcoma, the total dose of the drug was the same as above, and the tumors were administered locally and intraperitoneally at the same time. The tumor inhibition rates increased to 66.7% and 65.3%, respectively, demonstrating that the drug is effective against the above five tumors. Both species have inhibitory effects and increase the effect of topical administration. The LDS of the mice administered intraperitoneally to this preparation was determined herein. The mice were given a control of 90.7±4.9 mg/kgo per day for 1 week. The L, D so o for 6 consecutive days was 67.7±4.1 mg/kg. In vivo administration of the four types of sarcomas was performed for 14 days. The growth rate of both the drug and the control group: [] body mass (both detumor) was basically the same, and no significant toxicity was observed with this dose.