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目的:研究受精失败卵母细胞内精子解浓缩,卵子激活情况及纺锤体染色体分析,探讨补救性ICSI后受精失败的原因。方法:收集生殖中心行补救性ICSI后受精失败(受精率为0)的MII期卵母细胞,并分为早救性ICSI组和晚救性ICSI组,于取卵后48小时用免疫荧光法在激光共聚焦显微镜下观察。结果:收集95个受精失败的卵母细胞(其中早救性ICSI组46个,晚救性ICSI组49个),两组卵子激活障碍的发生率分别为60.9%,51%,没有统计学差异(P>0.05),卵子激活障碍的发生率远高于其他影响卵母细胞受精的因素的发生率。结论:卵子激活障碍是补救性ICSI后受精失败的最主要原因。
OBJECTIVE: To study the oocyte sperm solution concentration and egg activation in fertilized failed oocytes and chromosome analysis of spindle to explore the reasons for failed fertilization after ICSI. Methods: MII oocytes which failed in fertilization (fertilization rate of 0) after ICSI were collected and divided into early-salvage ICSI group and late-salvaged ICSI group. Immunocytochemistry Observed under a laser confocal microscope. RESULTS: Ninety-five oocytes failed to fertilize (46 in early-rescue ICSI group and 49 in ICSI group) were collected. The incidence of oocyte activation disorder in both groups was 60.9% and 51% respectively, with no statistical difference (P> 0.05), the incidence of oocyte activation disorder is much higher than the other factors that affect the oocyte fertilization rate. CONCLUSIONS: Oocyte activation disorder is the leading cause of failed fertilization after salvage ICSI.