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目的探讨核因子κB(NF-κB,p 6 5)在胰腺癌(PC)中的表达与淋巴结转移及MMP-2表达的关系。方法免疫印迹法检测4 5例PC(淋巴结转移3 0例,非转移1 5例)和9例正常胰腺组织(NP)中p 6 5蛋白的表达;RT-PCR检测MMP-2mRNA的表达。结果p 6 5,MMP-2在4 5例PC组织中的阳性表达率分别为6 6.7%(3 0/4 5)和5 7.8%(2 6/4 5),在3 0例转移组织中的阳性表达率分别为8 3.3%(2 5/3 0)和7 3.3%(2 2/3 0),在非转移组织中的阳性表达率分别为3 3.3%(5/1 5)和26.7%(4/1 5),p 6 5与MMP-2表达呈明显正相关(r=0.7 4 3,P<0.0 5)。结论NF-κB异常表达与胰腺癌淋巴结转移有关,并与MMP-2正相关,NF-κB可能通过对下游基因表达的调控,参与了胰腺癌的转移过程。
Objective To investigate the relationship between the expression of nuclear factor κB (NF-κB, p65) in pancreatic carcinoma (PC) and lymph node metastasis and the expression of MMP-2. Methods The expression of p65 protein in 45 cases of PC (lymph node metastasis in 30 cases, non-metastasis in 15 cases) and 9 cases of normal pancreas (NP) was detected by Western blotting. The expression of MMP-2 mRNA was detected by RT-PCR. Results The positive expression rate of MMP-2 in 45 cases of PC tissues was 6 6.7% (3 0/4 5) and 5 7.8% (2 6/4 5), respectively, in 30 cases of metastatic tissues The positive expression rates in non-metastatic tissues were 33.3% (5/1 5) and 26.7% respectively, which were 83.3% (2 5/3 0) and 7 3.3% (2 2/3 0), respectively % (4/1 5), p65 and MMP-2 were positively correlated (r = 0.743, P <0.05). Conclusion The abnormal expression of NF-κB is associated with lymph node metastasis of pancreatic cancer and positively correlated with MMP-2. NF-κB may be involved in the metastasis of pancreatic cancer through the regulation of downstream gene expression.