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应用发色底物法检测54例糖尿病(NIDDM)患者及30例健康人血浆组织型纤溶酶原激活物(t-PA)、纤溶酶原激活抑制物-1(PAI-1)及纤溶酶原(Plg)活性,放免法检测血清胰岛素,亲和层析法检测糖化血红蛋白(HbA1C)。结果显示:t-PA、P1g活性显著低于正常人(P<0.01及P<0.01),而PAI-1活性则显著高于正常人(P<0.01),HbA1C及胰岛素显著高于对照组(P<0.01及P<0.01);t-PA与PAI-1显著负相关(r=-0.445,P<0.01),t-PA与P1g呈正相关(r=0.294,P<0.05),PAI-1与胰岛素呈正相关(r=0.2493 P<0.05)。证明NIDDM患者可能有纤溶功能紊乱,且是致高凝倾向的原因之一,与糖尿病患者血管病变及血栓形成可能有一定相关性。糖尿病人PAI-1活性增强与HbA1C增高无关,与血清胰岛素水平正相关,提示胰岛素抵抗是导致PAI-1活性增高的主要原因之一。
The chromogenic substrate method was used to detect the plasma levels of tissue plasminogen activator (t-PA), plasminogen activator inhibitor-1 (PAI-1) and fibronectin in 54 patients with diabetes mellitus (NIDDM) Serum insulin was detected by radioimmunoassay and HbA1C was detected by affinity chromatography. The results showed that the activity of t-PA and P1g was significantly lower than that of normal people (P <0.01 and P <0.01), while PAI-1 activity was significantly higher than that of normal people (P <0.01), HbA1C and insulin were significantly higher (R = -0.445, P <0.01). There was a positive correlation between t-PA and P1g (r = 0.294, P <0.05) and PAI-1 And insulin was positively correlated (r = 0.2493 P <0.05). Proves NIDDM patients may have fibrinolytic disorders, and is one of the causes of the tendency to hypercoagulability, and diabetic patients with vascular lesions and thrombosis may have some relevance. PAI-1 activity in diabetic patients has nothing to do with the increase of HbA1C, but positively correlated with serum insulin level, suggesting that insulin resistance is one of the main reasons leading to the increase of PAI-1 activity.