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探讨恶性疟原虫保护性抗原复合基因 痘苗病毒重组活疫苗候选株在实验动物的抗疟原虫攻击能力 ,为下一步进入夜猴及人体试验奠定基础。方法 :利用多次部分换人血猕猴模经静脉输血法进行了抗疟原虫红内期虫体的攻击试验。结果 :在免疫后 1个月攻虫 ,重组痘苗病毒免疫猴从第 3天一直到第 12d的采血检查中均未发现疟原虫 ;非重组痘苗病毒(对照病毒 )免疫猴第 3天后原虫感染率上升 ,第 6天原虫感染率最高达到 6 .0 % ,而后原虫感染率逐渐下降 ,原虫持续时间为13天 ;空白对照猴第 3天后原虫感染率也上升 ,第 8天原虫感染率最高达到 2 .5 % ,而后原虫感染率逐渐下降 ,原虫持续出现时间为 12d。结论 :初步说明该候选疫苗株具有一定的抗恶性疟原虫红内期虫体攻击的能力。
To investigate the challenge ability of Plasmodium falciparum protective antigen composite gene vaccinia virus recombinant live vaccine candidate against Plasmodium in experimental animals and lay a foundation for the next step into the night monkey and human test. Methods: The challenge experiment of anti-parasite anti-malaria stage was carried out by means of intravenous transfusion with partial replacement of blood macaque. Results: Plasmodium was not found in the immunized monkeys vaccinated with recombinant vaccinia virus from the third day to the 12th day after vaccination. The infection rate of protozoal after 3 days of immunization with non-recombinant vaccinia virus (control virus) , The highest infection rate of protozoa reached 6.0% on the 6th day, while the infection rate of protozoa decreased gradually, the duration of protozoa was 13 days. The infection rate of protozoa on the 3rd day of blank control monkey also increased, .5%, while the protozoal infection rate gradually decreased, protozoan continued to appear for 12 days. CONCLUSIONS: This preliminary study indicates that the candidate vaccine strain possesses the certain ability of attacking against parasite Plasmodium falciparum.