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１９９５年广东省番禺等市发生登革热流行，应用间接免疫荧光试验检测５８份待测登革ＩｇＧ的标本，３８份阳性，阳性率６５．５２％（３８／５８）；检测疑似登革热患者早期血标本５１份，用Ｃ６／３６细胞分离出２８株登革Ｉ型病毒，阳性率５４．９０％（２８／５１）．证实本次流行是登革Ｉ型病毒所引起，二者检出率差异无显著性（Ｘ２＝１．２８，Ｐ＞０．０５）。ＩｇＧ最早检出于发病后第三天，一周后达高峰，患者血清中各型登革ＩｇＧ呈高度交叉反应，不能分型，其平均几何滴度在１：１００以上。此外，ＩＥＡ检测确诊登革病人血清９份。ＩｇＧ阳性率为１００％，滴度范围为１：１６０－１：１２８０：检测非登革血清３３６份，滴度１：４０有１５份，１：８０有１份，阳性率为４．７６％。此法检测登革ＩｇＧ，方法简单、敏感、快速、经济。结果表明：病后一周取检，ＩｇＧ抗体滴度达１：８０以上，对诊断为登革热感染有参考意义。
Dengue epidemic occurred in Panyu and other cities of Guangdong Province in 1995. Indirect immunofluorescence assay was used to detect 58 specimens of Dengue IgG to be tested, 38 were positive and the positive rate was 65.52% (38/58). The detection of early blood samples in suspected dengue patients Twenty-eight dengue virus type I viruses were isolated from C6 / 36 cells with a positive rate of 54.90% (28/51). Confirmed that the epidemic is caused by dengue type I virus, the detection rate was no significant difference between the two (X2 = 1.28, P> 0.05). The earliest detection of IgG was on the third day after onset and peaked one week later. All kinds of dengue IgG in serum were highly cross-reactive and could not be typed. The average geometric titers were above 1: 100. In addition, IEA detected 9 serum samples of dengue patients. IgG positive rate of 100%, titer range of 1: 160-1: 1280: detection of non-dengue 336, titer 1:40 15, 1: 80 1, the positive rate was 4.76% . Dengue IgG detection method, the method is simple, sensitive, fast and economical. The results showed that one week after the illness, the IgG antibody titers reached more than 1:80, which is of reference value for the diagnosis of dengue infection.