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采用分子原位杂交和ＳＰ法免疫组化技术对柳州地区４０例食管鳞状细胞癌和２０例食管粘膜慢性炎的材料进行地高辛标记的ＨＰＶ６Ｂ／１１、１６、１８ＤＮＡ及ＨＰＶ１６、１８早期蛋白Ｅ６、Ｐ２１ｒａｓ和Ｐ５３癌基因产物检测，结果显示：食管癌中ＨＰＶ１６、１８ＤＮＡ及Ｅ６蛋白的阳性率分别为２５％（１０／４０）和６５％（２６／４０），与对照组对比差异均有高度显著性（Ｐ＜０．０１）。Ｅ６、和Ｐ５３蛋白呈双阳性者为５２．５０％（２１／４０），其中９０．４８％（１９／２１）阳性表达出现在同一区域同一癌细胞核内，似表达Ｅ６、可与Ｐ５３结合形成复合物，从而导致野生型Ｐ５３的降解或突变。本组鳞癌组织中Ｐ２１ｒａｓ与Ｐ５３、Ｐ５３与Ｅ６的阳性表达均具有显著相关性（Ｐ＜０．０５）。提示高危ＨＰＶ１６、１８感染与多癌基因协同在本地区食管癌病因学中起着十分重要的作用。
Digoxin-labeled HPV6B/11,16,18 DNA and HPV16,18 early protein in 40 cases of esophageal squamous cell carcinoma and 20 cases of chronic inflammation of esophageal mucosa in Liuzhou using molecular in situ hybridization and SP immunohistochemistry The detection of E6, P21ras and P53 oncogene products showed that the positive rates of HPV16, 18DNA and E6 in esophageal cancer were 25% (10/40) and 65% (26/40), respectively, and there were significant differences compared with the control group. Highly significant (P<0.01). The positive expression rate of E6 and P53 were 52.50% (21/40), of which 90.48% (19/21) positive expression appeared in the same cell nucleus in the same region, and it seemed to express E6, which could be combined with P53. Complexes, which lead to degradation or mutation of wild-type P53. The positive expression of P21ras and P53, P53 and E6 in this group of squamous cell carcinomas were significantly correlated (P<0.05). It suggests that high-risk HPV16, 18 infection and multi-cancer genes play an important role in the etiology of esophageal cancer in this region.