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【目的】通过基于1H-NMR的代谢组学方法检测早产儿并发呼吸窘迫综合征(neonatal respiratory distresssyndrome,NRDS)的代谢产物,探讨代谢组学在早产儿疾病中的应用及寻找早产儿NRDS的早期生物标志物(群)。【方法】分为早产儿NRDS组和早产儿肺炎组,每组各20例;2组在入院年龄、胎龄、出生体重等方面具有可比性。所有病例于入院半小时内抽血、收集血浆,用1H-NMR方法检测血浆中的小分子物质和脂类代谢物。【结果】1)小分子物质分析:NRDS组与肺炎组相比,血浆中乳酸、某种脂质及乙醇增加,而葡萄糖、肌酸、谷氨酰胺、O-乙酰基(OAc)、N-乙酰基(NAc)、丙氨酸、缬氨酸、异亮氨酸降低;2)脂质分析:NRDS组与肺炎组相比,血浆中带HC=CH基团脂质、NAc、磷脂酰胆碱(PtdCho)、低密度脂蛋白(LDL)和极低密度脂蛋白(VLDL)降低。【结论】1)NRDS早产儿存在乳酸的堆积、主要供能物质葡萄糖及重要氨基酸等的减少;2)NRDS早产儿存在脂质代谢紊乱;3)代谢组学方法在早产儿疾病中有较大的应用前景。
【OBJECTIVE】 Metabolomics was used to detect the metabolites of neonatal respiratory distress syndrome (NRDS) in preterm infants based on 1H-NMR and to explore the application of metabolomics in premature infants and the early stage of NRDS search Biomarkers (groups). 【Methods】 Divided into preterm infants with NRDS group and premature infantile pneumonia group, each group 20 cases; 2 groups in admission age, gestational age, birth weight and other aspects comparable. In all cases, blood samples were collected within half an hour of admission, plasma was collected, and small molecules and lipid metabolites in plasma were detected by 1H-NMR. 【Results】 1) Analysis of small molecules: Compared with pneumonia group, NRDS group showed increased plasma lactic acid, some lipid and ethanol, while glucose, creatine, glutamine, OAc, N- (NAc), alanine, valine and isoleucine decreased; 2) Lipid analysis: Compared with the pneumonia group, the NRDS group had higher HC = CH group lipid, NAc, phosphatidylcholine PtdCho, LDL and VLDL decreased. 【Conclusion】 1) There is lactic acid accumulation in premature infants with NRDS, the decrease of glucose and important amino acids, etc .; 2) Premature infants with prenatal NRDS have dyslipidemia; 3) Metabolomics is more prevalent in preterm infants The application prospects.