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目的 研究大鼠不同发育阶段视皮层神经元电生理学与形态学特性的关系 ,观察电生理特性和形态变化的同步化程度 ,认识正常视觉发育的细胞内机制。方法 应用脑片膜片钳全细胞记录技术和细胞内标记相结合的方法 ,获得 4～ 2 8d Sprague- Dawleg(SD)大鼠视皮层神经元的细胞内微电极记录 ,再进行细胞内标记和组织学染色。 结果 成功标记 2 3例细胞 ,其中锥体细胞与非锥体细胞在电生理特性上差异有显著性。不同发育阶段视皮层神经元形态学上的成熟度不同。 结论 1视觉发育过程中 ,视皮层锥体细胞和非锥体细胞的电生理学特性反映其参与不同的视皮层神经元回路的整合功能。 2视觉发育可塑性关键期内 ,视皮层神经元形态和电生理特性变化的同步化程度大于视皮层下结构。
Objective To study the relationship between the electrophysiological and morphological characteristics of the visual cortex neurons in different developmental stages of rats and to observe the synchronization of electrophysiological characteristics and morphological changes and to understand the intracellular mechanism of normal visual development. Methods The intracellular microelectrode recording of neurons in the visual cortex of 4 ~ 28-day Sprague-Dawleg (SD) rats was obtained by the combination of whole-cell patch-clamp technique and intracellular labeling. Then intracellular microelectrode recording Histological staining. Results Twenty-three cells were successfully labeled, of which pyramidal cells and non-pyramidal cells were significantly different in electrophysiological characteristics. Different stages of development of visual cortex neurons of different maturity. Conclusions1 The electrophysiological characteristics of visual cortex pyramidal cells and non-pyramidal cells during visual development reflect their integration function in different visual cortex neurons. 2 visual development plasticity key period, visual cortex neurons and electrophysiological changes in the degree of synchronization is greater than the visual cortex structure.