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目的采用小鼠移植瘤模型,对金黄葡萄球菌肠毒素C2的抗肿瘤作用进行研究,以进一步揭示超抗原在肿瘤治疗方面的意义。方法从金葡菌发酵液中提纯SEC2蛋白,并经SDS-PAGE及测序分析;构建小鼠S180肉瘤和Lewis肺癌移植瘤模型,分别腹腔注射高、中、低剂量(800、400、200 ng/kg)的SEC2,连续给药10 d,同时以环磷酰胺为阳性对照,生理盐水为阴性对照。于给药后第11天处死小鼠,将肿瘤组织分离并称重,比较SEC2对S180肉瘤及Lewis肺癌移植瘤的抑瘤效果。结果在相对分子质量约30 000处可见目的条带,与理论值相符,N-未端氨基酸测序与文献报道一致;SEC2对两种移植瘤均产生较明显抑制作用,高、中、低剂量SEC2对S180肉瘤的抑瘤率分别达到50.60%、31.81%和19.38%,对Lewis肺癌的抑瘤率分别达到55.62%、45.70%和37.78%。结论从动物水平上确认了SEC2的抑瘤效果,为进一步开展超抗原抗肿瘤研究奠定了基础。
Objective To study the antitumor effect of staphylococcal enterotoxin C2 in mice transplanted tumor model to further reveal the significance of superantigen in the treatment of cancer. Methods SEC2 protein was purified from S. aureus fermentation broth and analyzed by SDS-PAGE and sequencing. Mouse S180 sarcoma and Lewis lung carcinoma xenograft model were established and injected intraperitoneally with high, medium and low dose (800, 400, 200 ng / kg) of SEC2, continuous administration of 10 d, while cyclophosphamide as a positive control, normal saline as a negative control. Mice were sacrificed on the 11th day after administration, the tumor tissues were isolated and weighed, and the antitumor effect of SEC2 on S180 sarcoma and Lewis lung carcinoma was compared. Results The target band was found at molecular weight of about 30 000, consistent with the theoretical value. N-terminal amino acid sequencing was consistent with that reported in the literature. SEC2 had a significant inhibitory effect on both xenografts, with high, medium and low doses of SEC2 The antitumor rates of S180 sarcoma reached 50.60%, 31.81% and 19.38%, respectively, and the inhibitory rates against Lewis lung cancer were 55.62%, 45.70% and 37.78%, respectively. Conclusions The anti-tumor effect of SEC2 was confirmed from animal level, which lays the foundation for further research on anti-tumor of superantigen.