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Objective:To evaluate the anti-bacterial and anti-oxidant activity of and rographolide(AND)and echiodinin(ECH)of Andrographis paniculata.Methods:In this study,an attempt has been made to demonstrate the anti-microbial and anti-oxidant activity of isolated AND and ECH by broth micro-dilution method and 2,2-dipheny l-2-picryl-hydrazyl(DPPH)assay,respectively.Structure elucidation was determined by electro-spray ionization-MSD,NMR(1H and13C)and IR spectra.Results:AND was effective against most of the strains tested including Mycobacterium smegmatis,showing broad spectrum of growth inhibition activity with Minimum inhibitory concentration values againstStaphylococcus aureus(100μg/mL),Streptococcus thermophilus(350μg/mL)Bacillus subtilis(100μg/mL),Escherichia coli(50μg/mL),Mycobacterium smegmatis(200μg/mL),Klebsiella pneumonia(100μg/mL),andPseudomonas aeruginosa(200μg/mL).ECH showed specific anti-bacterial activity against Staphylococcus aureus,Escherichia coli,Bacillus subtilis and Pseudomonas aeruginosaat a concentration higher than 225μg/mL.Both AND and ECH were not effective against the two yeast strains,Candida albicansand Saccharomyces cerevisiae tested in this study.Conclusion:This preliminary study showed promising anti-bacterial activity and moderate free radical scavenging activity of AND and ECH,and it may provide the scientific rationale for its popular folklore medicines.
Objective: To evaluate the anti-bacterial and anti-oxidant activity of and rographolide (AND) and echiodinin (ECH) of Andrographis paniculata. Methods: an this has been demonstrated to the anti-microbial and anti-oxidant activity of isolated AND and ECH by broth micro-dilution method and 2,2-dipheny l-2-picryl-hydrazyl (DPPH) assay, respectively. Structure elucidation was determined by electro-spray ionization- MSD, NMR (1H and13C) and IR spectra.Results: AND was effective against most of the strains tested including Mycobacterium smegmatis, showing broad spectrum of growth inhibition activity with Minimum inhibitory concentration values againstStaphylococcus aureus (100 μg / mL), Streptococcus thermophilus (350 μg / mL) ), Escherichia coli (50 μg / mL), Mycobacterium smegmatis (200 μg / mL), Klebsiella pneumonia (100 μg / mL), and Pseudomonas aeruginosa (200 μg / mL) .ECH showed specific anti-bacterial activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosaat a concentration higher than 225 μg / mL.Both AND and ECH were not effective against the two yeast strains, Candida albicans and Saccharomyces cerevisiae tested in this study. Conlusion: This preliminary study marked promising anti-bacterial activity and moderate free radical scavenging activity of AND and ECH, and it may provide the scientific rationale for its popular folklore medicines.