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目的探讨CD34在人肝细胞肝癌(HCC)组织中的表达及临床意义。方法对54例HCC及相应的癌旁肝组织标本进行了CD34免疫组织化学EnVisionTM法检测;对CD34标记的阳性血管进行肿瘤微血管密度(MVD)计数,分析临床病理特征。结果 CD34呈棕黄色染色定位于血管内皮细胞膜上,在HCC组织内显示弥漫的阳性表达;而癌旁肝组织多为阴性。HCC组织MVD值(46.23±16.12),明显高于癌旁肝组织的(18.15±5.92)(P<0.01)。HCC中CD34表达与EdmonsongradeⅢ+Ⅳ、静脉浸润、肝内外转移、肝硬化有关(P<0.05)。结论 HCC中CD34异常高表达,且与部分侵袭转移指标相关。
Objective To investigate the expression of CD34 in human hepatocellular carcinoma (HCC) and its clinical significance. Methods 54 cases of HCC and its corresponding adjacent normal liver tissues were examined by CD34 immunohistochemistry EnVisionTM method. The number of CD34 positive vessels was counted by microvessel density (MVD), and the clinicopathological features were analyzed. Results CD34 was located on the vascular endothelial cell membrane with brown staining, which showed diffuse positive expression in HCC tissues. However, the paracancer liver tissues were mostly negative. The MVD value of HCC tissues was (46.23 ± 16.12), which was significantly higher than that of adjacent non-cancerous liver tissues (18.15 ± 5.92) (P <0.01). CD34 expression in HCC was associated with EdmonsongradeⅢ + Ⅳ, venous invasion, extrahepatic metastasis and cirrhosis (P <0.05). Conclusion The abnormal expression of CD34 in HCC is closely related to some indicators of invasion and metastasis.