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用Cu~(2+)(引发氧化修饰)和脂质过氧化降解产物丙二醛对低密度脂蛋白(LDL)进行修饰,分别测定了巨噬细胞系P~(300)D_1和小鼠腹腔巨噬细胞对两种被修饰LDL的结合量(包括内移量)和降解量。结果显示:LDL经氧化修饰和丙二醛修饰后被两类巨噬细胞的结合量与降解量均高于正常LDL,在修饰程度相近(琼脂糖电泳迁移率相近)时,两类巨噬细胞对氧化修饰LDL的结合量与降解量高于丙二醛修饰的LDL。竞争性抑制结果显示,丙二醛修饰的LDL和乙酰化修饰的LDL均可部分抑制巨噬细胞对氧化修饰LDL的结合与降解。
Low density lipoprotein (LDL) was modified by Cu ~ (2 +) (priming oxidation) and malondialdehyde (MDA), the peritoneal cavity of macrophage cell line P ~ (300) D_1 The amount of macrophages bound to both modified LDLs, including the amount of internal migration, and the amount of degradation. The results showed that the binding amount and degradation amount of LDL by both oxidative modification and malondialdehyde modification were higher than those of normal LDL. When the degree of modification was similar (agarose electrophoresis mobility was similar), two types of macrophages The amount and degradation of oxidized modified LDL was higher than that of malondialdehyde modified LDL. Competitive inhibition results show that both malondialdehyde-modified LDL and acetylated modified LDL can partially inhibit the binding and degradation of macrophages to oxidized LDL.