磷脂酰肌醇合成酶PIS基因植物表达载体的构建

来源 :山东林业科技 | 被引量 : 0次 | 上传用户：ydaf5hv2

【摘要】

：

磷脂酰肌醇(PI)是真核细胞中主要的磷脂组分,磷脂酰肌醇合成酶(PIS)是磷脂酰肌醇合成途径中的关键酶,它的功能是催化myo一肌醇和CDP-DG反应产生磷脂酰肌醇.将玉米PIS基因与载

【作者】

：

庞彩红咸洋夏阳李双云刘翠兰燕丽萍李丽杨青山王

【机构】

：

山东省林业科学研究院

【出处】

：

山东林业科技

【发表日期】

：

2004年期

【关键词】

：

PIS基因植物表达载体构建

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磷脂酰肌醇(PI)是真核细胞中主要的磷脂组分,磷脂酰肌醇合成酶(PIS)是磷脂酰肌醇合成途径中的关键酶,它的功能是催化myo一肌醇和CDP-DG反应产生磷脂酰肌醇.将玉米PIS基因与载体pCAMBIA2300-35s连接,得到植物表达载体pCAMBIA2300-35s-PIS,该载体带有CaMV35s启动子、PIS基因、终止序列及一个CaMV35s启动子启动的NPTⅡ抗性筛选基因.将构建好的PIS植物表达载体转入农杆茵中,用于将来国槐等木本植物的转化.

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磷脂酰肌醇合成酶PIS基因植物表达载体的构建

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