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姜荷花是国内新兴的一种热带花卉,但中国绝大部分地区冬季寒冷导致种球不能自然越冬保存。为了明确姜荷花‘玉如意’种球的抗寒生理、并找出提升种球抗寒性的方法。本研究对经不同低温胁迫处理‘玉如意’种球的各种生理生化指标进行了检测,并开展了种球的抗寒药剂筛选试验。种球抗寒性生理生化指标检测试验表明,‘玉如意’种球在9℃时开始受逆境胁迫;根据抗氧化酶系统和渗透调节系统的变化可知,在5℃和1℃时种球调动了自身的抗逆系统抵御环境的不良影响;而在-3℃时,各抗逆相关指标基本上降低到最低点,超出了种球的耐受极限。抗寒药剂筛选试验表明,各药剂处理(包括脱落酸(ABA),水杨酸(SC),芸苔素内酯(BR),6-BA,多效唑(PP333)等)对增强种球的抗寒性都有一定的作用。根据细胞受损程度分析,ABA、6-BA和PP333处理对种球的保护效果较好。ABA主要通过调整渗透调节系统来增加种球抗寒性,而6-BA则主要是通过调节抗氧化酶系统来达到目的。SC和BR处理的抗寒效果稍差。
Ginger lotus is a newly emerging tropical flower in China, but the winter cold in most parts of China caused the bulbs to fail to survive the winter. In order to clarify the ginger lotus ’jade wish’ planktonic cold physiology, and to find ways to enhance the cold hardiness of bulbs. In this study, various physiological and biochemical indexes of ’Yuruyi’ bulbs treated by different low temperature stress were tested, and the screening test of cold-resistant bulbs of bulbs was carried out. Physiological and biochemical indexes of cold-resistant bulbs showed that ’Yuruyi’ bulbs began to be under stress at 9 ℃. According to the changes of antioxidant enzyme system and osmotic adjustment system, bulbs transferred at 5 ℃ and 1 ℃ Its own anti-retrograde system to withstand the adverse effects of the environment; and at -3 ℃, the anti-stress related indicators basically reduced to the lowest point, beyond the tolerance of bulbs. Cold-resistant agent screening test showed that the effect of each agent treatment (including ABA, SC, BR, 6-BA, PP333) Cold sex has a certain effect. According to the degree of cell damage analysis, ABA, 6-BA and PP333 treatment of seed ball protection effect is better. ABA mainly increases the cold tolerance of bulbs by adjusting the osmotic adjustment system, while 6-BA achieves its goal mainly through regulating the antioxidant enzyme system. Cold treatment of SC and BR slightly less effective.