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目的:探讨中药复方糖络宁对糖尿病大鼠坐骨神经氧化应激及超微结构的影响。方法:SD大鼠55只,随机选取12只为正常对照组,余43只大鼠予一次性腹腔注射STZ建立糖尿病大鼠模型,造模成功后随机分为模型组、糖络宁组、α-硫辛酸组。两给药组干预4个月后,用透射电镜观察各组大鼠坐骨神经的超微结构,检测坐骨神经丙二醛(MDA)含量,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活力和半胱天冬酶3(Caspase-3)活性。结果:与正常对照组相比,模型组的MDA含量和Caspase-3活性明显升高(P<0.05),SOD、CAT的活力明显下降(P<0.05)。与模型组相比,糖络宁组和α-硫辛酸组的MDA含量和Caspase-3活性明显下降(P<0.05),SOD、CAT的活力明显升高(P<0.05)。糖络宁组的MDA含量、Caspase-3活性和SOD、CAT的活力,与α-硫辛酸组相比差异无统计学意义。透射电镜观察坐骨神经超微结构显示模型组部分纤维髓鞘增厚或松解,轻度轴索变性。糖络宁组仅见少数纤维髓鞘松解,病变明显轻于模型组。结论:中药复方糖络宁可能通过提高SOD、CAT的活力、清除脂质氧化终产物MDA,减轻糖尿病周围神经病变(DPN)的过氧化损伤,减少坐骨神经细胞凋亡,延缓DPN的进展。
Objective: To investigate the effect of Tangluoning, a traditional Chinese medicine compound, on oxidative stress and ultrastructure of sciatic nerve in diabetic rats. Methods: Fifty-five SD rats were selected randomly from 12 rats as normal control group and the other 43 rats were given intraperitoneal injection of STZ once a day to establish diabetic rat models. After successful modeling, they were randomly divided into model group, - Lipoic acid group. After intervention for 4 months, the ultrastructure of sciatic nerve in each group was observed by transmission electron microscopy. The contents of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) Viability and caspase-3 activity. Results: Compared with the normal control group, the content of MDA and the activity of Caspase-3 in the model group were significantly increased (P <0.05), and the activities of SOD and CAT were significantly decreased (P <0.05). Compared with the model group, the content of MDA and the activity of Caspase-3 in the meloxicam group and the α-lipoic acid group decreased significantly (P <0.05), and the activities of SOD and CAT increased significantly (P <0.05). The content of MDA, the activities of Caspase-3 and the activities of SOD and CAT in the sugar-regulating group were not significantly different from those in the α-lipoic acid group. Transmission electron microscopy showed that the sciatic nerve ultrastructure showed thickening or loosening of myelin sheath and mild axonal degeneration in the model group. In the meloxicam group, only a few fibrinolytic myelin sheaths were found, and the lesions were significantly lighter than the model group. CONCLUSION: Tangluoning, a traditional Chinese drug compound, may delay the DPN progression by increasing the activity of SOD and CAT, clearing the lipid oxidation end product MDA, reducing the peroxidative damage of diabetic peripheral neuropathy (DPN) and reducing the apoptosis of sciatic nerve cells.