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应用免疫组化ＡＢＣ法检测１００例甲状腺乳头状癌标本中ｎｍ２３基因产物二磷酸核苷激酶（ＮＤＰＫ）的表达。结果总阳性率６７％，其中无淋巴结转移组阳性率８５％（３４／４０），伴有淋巴结转移组阳性率５５％（３３／６０）（Ｐ＜０．０１）；未侵犯包膜组阳性率７９％（３０／３８），侵犯包膜组阳性率６０％（３７／６２）（Ｐ＜０．０５），提示ｎｍ２３基因在甲状腺乳头状癌的表达与淋巴结转移及包膜侵犯呈负相关关系。结果还显示，在ｎｍ２３基因表达阳性的病例中，未侵犯包膜与侵犯包膜组淋巴结转移率分别是７％（２／３０）及８４％（３１／３７），（Ｐ＜０．０１），提示尽管ｎｍ２３阳性，侵犯包膜组明显比未侵犯包膜组淋巴结转移机会大；在未侵犯包膜的病例中，ｎｍ２３（－）及ｎｍ２３（＋）病例的淋巴结转移率分别是７５％（６／８）及７％（２／３０）（Ｐ＜０．０５），提示尽管未侵犯包膜，但在ｎｍ２３阴性时，淋巴结转移的可能性仍很大。结论：本检测有助于探讨代表甲状腺乳头状癌转移趋势的分子生物学标志，更科学地选择手术方式。
Immunohistochemical ABC method was used to detect the expression of nm23 gene NDPK in 100 samples of thyroid papillary carcinoma. Results The positive rate was 67%. The positive rate of non-lymph node metastasis was 85% (34/40), the positive rate of lymph node metastasis was 55% (33/60) (P <0.01) (79/62), and the positive rate of encrusting group was 60% (37/62) (P <0.05), which suggested that the expression of nm23 in thyroid papillary carcinoma was negatively correlated with lymph node metastasis and invasion relationship. The results also showed that lymph node metastasis rate was 7% (2/30) and 84% (31/37) respectively in the cases with positive expression of nm23 gene (P <0.01) , Suggesting that although nm23 was positive, the invasion of the capsule group was significantly greater than the non-invasive capsule lymph node metastasis; in non-invasive capsule cases, nm23 (-) and nm23 (+) cases of lymph node metastasis rates were 75% 6/8), and 7% (2/30) (P <0.05), suggesting that there is still a high probability of lymph node metastasis when nm23 is negative, although the envelope is not infiltrated. Conclusion: This test is helpful to explore molecular biology markers that represent the trend of metastasis of thyroid papillary carcinoma and choose the surgical method more scientifically.