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目的:探索中药复方对Uu感染的非淋菌性尿道炎(NGU)的作用机理,从多角度为中医药治疗本病提供理论依据。方法:63个Uu临床分离株采用肉汤稀释法判定临床耐红霉素和四环素(MIC≥16μg/ml,参照美国国家临床实验室标准委员会1995年标准);经三次传代成纯培养后接种于96孔板小孔中,药液倍比稀释成250mg/ml~0.48mg/ml,37℃培养,加药组以72h后仍不出现培养基由黄→红色变化的最小药物浓度为该药的最小抑菌浓度(MIC)。结果:尿路清对63株Uu临床株MIC范围为0.48mg/ml~15.36mg/ml,MIC50≤1.95mg/ml,MIC90≤3.91mg/ml,Uu临床株对尿路清的敏感性比对四环素的敏感性高的多(X2=18.38,P<0.01),Uu临床株对尿路清的敏感性与对红霉素的敏感性之间无明显联系(X2=1.85,P<0.05)。结论:中药尿路清对63株耐药(四环素、红霉素)菌株Uu显示出了较强的体外抑菌作用,值得进一步研究。
Objective: To explore the mechanism of traditional Chinese medicine on Uu infection of non-gonococcal urethritis (NGU) mechanism, from multiple perspectives for the treatment of this disease provides a theoretical basis. Methods: Sixty-three clinical isolates of Uu were tested for clinical resistance to erythromycin and tetracycline using the broth dilution method (MIC≥16μg / ml, according to the standards of the National Committee for Clinical Laboratory Standards of the United States of 1995). After three subcultures into pure culture, 96-well plates in the hole, the ratio of drug solution diluted to 250mg / ml ~ 0.48mg / ml, 37 ℃ culture, the drug group 72h after the medium does not appear the change from yellow to red minimum drug concentration of the drug Minimum inhibitory concentration (MIC). Results: The urinary clearance of 63 clinical strains of Uu MIC range of 0.48mg / ml ~ 15.36mg / ml, MIC50 ≤ 1.95mg / ml, MIC90 ≤ 3.91mg / ml, Uu clinical strains on the sensitivity of urinary tract clearance The sensitivity of tetracycline to tetracycline was much higher (X2 = 18.38, P <0.01). There was no significant correlation between the sensitivity of Uu to urinary tract clearance and erythromycin sensitivity (X2 = 1.85, P <0.05). Conclusion: Urease of traditional Chinese medicine has a strong antibacterial activity against 63 strains of Uu (tetracycline and erythromycin) strains in vitro, which deserves further study.