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目的:采用近红外光谱法对温郁金中吉马酮含量进行快速测定。方法:以高效液相色谱(HPLC)分析值作为参照,采用傅里叶变换近红外漫反射光谱技术采集温郁金的近红外光谱,结合偏最小二乘法(PLS)建立吉马酮含量的快速测定方法。结果:吉马酮校正模型的相关系数(r)、校正均方差(RM-SEC)、内部验证均方差(RMSEP)分别为0.998 65,0.006 91,0.002 41。经外部验证,预测值与实测值的相关系数达0.999 4。结论:该方法准确、快速、简便,可实现大批量样品的快速分析。
Objective: To determine the content of gemfurizan in warm turmeric by near infrared spectroscopy. METHODS: Near-infrared spectra of warm turmeric were collected by Fourier transform near-infrared diffuse reflectance spectroscopy (HPLC) with high performance liquid chromatography (HPLC) analysis. The rapid determination of gemcitabine . Results: Correlation coefficient (r), corrected mean square error (RM-SEC) and mean square error of internal validation (RMSEP) of the calibration model were 0.998 65,0.006 91,0.002 41 respectively. External validation, the predicted value and the measured value of the correlation coefficient of 0.999 4. Conclusion: The method is accurate, rapid and simple, and can be used to rapidly analyze large quantities of samples.