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目的:探讨普萘洛尔对脂多糖诱导BALB/C小鼠腹膜巨噬细胞分泌肿瘤坏死因子(TNF)-α和白细胞介素(IL)-10以及吞噬功能的影响。方法:将腹膜巨噬细胞分为8组:空白组,脂多糖组,普萘洛尔(0.1μmol/L)组,普萘洛尔(1.0μmol/L)组,普萘洛尔(10.0μmol/L)组;普萘洛尔(0.1μmol/L)加脂多糖组,普萘洛尔(1.0μmol/L)加脂多糖组,普萘洛尔(10.0μmol/L)加脂多糖组。在培养6h和24h后用酶联免疫法测定各培养瓶上清液中TNF-α和IL-10的浓度,用中性红吞噬试验检测巨噬细胞吞噬功能。结果:空白组与单用普萘洛尔时,巨噬细胞均无细胞因子分泌(无数据显示),吞噬功能无明显影响。单用脂多糖组巨噬细胞释放TNF-α和IL-10显著增加,吞噬能力明显增强。而普萘洛尔加脂多糖组巨噬细胞继续分泌TNF-α,IL-10分泌减少,吞噬功能显著降低,该作用随普萘洛尔浓度增加而增强。结论:普萘洛尔可调节腹膜巨噬细胞炎症递质的分泌,并能影响腹膜巨噬细胞的吞噬能力。
Objective: To investigate the effect of propranolol on lipopolysaccharide-induced secretion of tumor necrosis factor (TNF) -α and interleukin (IL) -10 and phagocytosis of peritoneal macrophages in BALB / C mice. Methods: Peritoneal macrophages were divided into 8 groups: blank group, lipopolysaccharide group, propranolol 0.1μmol / L group, propranolol 1.0μmol / L group, propranolol 10.0μmol / L group; propranolol (0.1μmol / L) plus lipopolysaccharide group, propranolol (1.0μmol / L) plus lipopolysaccharide group, propranolol (10.0μmol / L) plus lipopolysaccharide group. The concentrations of TNF-α and IL-10 in each culture flask supernatant were determined by enzyme-linked immunosorbent assay (ELISA) at 6h and 24h after culture, and phagocytosis of macrophages was detected by neutral red phagocytosis assay. Results: There were no cytokine secretion in macrophages (no data) and phagocytosis in blank group and propranolol alone. TNF-αand IL-10 released by macrophages in LPS group were significantly increased, phagocytosis was significantly enhanced. The propranolol plus lipopolysaccharide group of macrophages continue to secrete TNF-α, IL-10 secretion decreased, phagocytic function was significantly reduced, the effect increased with increasing concentrations of propranolol. Conclusion: Propranolol can regulate the secretion of inflammatory mediators of peritoneal macrophages and affect the phagocytic capacity of peritoneal macrophages.