如何快速、简便、准确地测定种子发芽能力,历来为人们所重视。因为种子发芽能力在留种入库、种子调运、贮藏交换以及大田播种时,均是评价待用种子质量的重要指标。常规测定方法,不仅所需测定时间长,尤其难以分辨逾期不发芽的种子是丧失了发芽能力?还是休眠的表现?故对于那些新收种子的测定结果,其可靠度甚差。红四唑染色法虽然能够克服常规测定法的缺点,但由于油菜籽小皮薄,测定时不仅去除种皮繁杂费时,而且容易肢解种胚的4个部分,更因在染色图形的经验判断上,每个观察者所掌握的标准不一,会出现测定结果上的较大误差。为解决上述问题,我们在联合国粮农组织国际植物遗传资源委 How to quickly, easily and accurately determine the seed germination capacity has always been valued by people. Because seed germination ability to stay in storage, seed transfer, storage and exchange and sowing, evaluation of the quality of the seeds are used as an important indicator. Routine determination methods, not only the required measurement time is long, especially difficult to tell whether overdue non-germinating seeds is the loss of germination capacity or the performance of the hibernation of the newly-seeded results, the reliability of the results is poor. Although the red tetrazolium staining method can overcome the shortcomings of conventional methods, but because of small rapeseed thin skin, determination of not only remove the seed coat is complicated and time-consuming, but also easy to dismember the embryo of the four parts, but also because of the staining pattern of the experience of judgment , Each observer mastered the different standards, there will be a larger measurement error. In order to solve the above problems, we are at the International Plant Genetic Resources Committee of the Food and Agriculture Organization of the United Nations