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目的 研究突变型ｐ５３基因在人胃癌组织中的表达及临床意义。方法分别应用ＰＣＲ－ＳＳＣＰ及原位分子杂交技术， 检测４１例胃癌组织和２６例癌旁非病变粘膜ｐ５３基因点突变及ｍＲＮＡ过表达情况。结果人胃癌组织存在ｐ５３基因突变和 ｍＲＮＡ过表达率明显高于癌旁非病变粘膜（Ｐ＜０．０５）。肿瘤组织分化越差，ｐ５３突变率越高，ｍＲＮＡ过表达的分布越广泛（Ｐ＜ ０．０５）。发生ｐ５３基因突变的胃癌病人较未发生者更易发生淋巴结转移（Ｐ＜０．０５）。结论胃癌的发生、发展与ｐ５３基因突变有 关，检测胃癌组织中ｐ５３突变情况可为胃癌的诊断、分期及预后评估提供可靠依据。
Objective To study the expression and clinical significance of mutant p53 gene in human gastric cancer. Methods PCR-SSCP and in situ hybridization were used to detect p53 gene mutation and mRNA overexpression in 41 cases of gastric cancer and 26 cases of non-cancerous adjacent mucosa. Results The p53 gene mutation and mRNA overexpression in gastric cancer tissues were significantly higher than those in adjacent non-lesion mucosa (P <0.05). The worse the tumor tissue differentiation, the higher the mutation rate of p53, the more widespread the overexpression of mRNA (P <0.05). The occurrence of p53 gene mutation in gastric cancer patients were more likely to occur than in patients with lymph node metastasis (P <0.05). Conclusion The occurrence and development of gastric cancer are related to the mutation of p53 gene. Detecting the mutation of p53 in gastric cancer can provide a reliable basis for the diagnosis, staging and prognosis of gastric cancer.