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本文应用免疫组化及ＰＣＲ－ＳＳＣＰ技术对５８例乳腺肿瘤ｃ－ｅｒｂＢ－２癌基因表达及ｐ５３抑癌基因突变进行研究，结果显示乳腺癌中ｃ－ｅｒｂＢ－２表达率为３８％，ｐ５３表达率为５２％，导管内乳头状瘤均为阴性；ｃ－ｅｒｂＢ－２表达与乳腺癌组织学分级（Ｐ＜０．０１）及淋巴结转移呈负相关（Ｐ＜０．０１），与雌激素受体状态呈正相关（Ｐ＜０．０１）；ｐ５３表达与淋巴结转移呈正相关（Ｐ＜０．０１），且倾相于≥２ｃｍ的肿瘤；两基因的表达均与乳腺上皮细胞的恶性转化及淋巴结转移密切相关，但两基因间无明显相关性；因此，每一基因的改变均可作为不依赖于淋巴结状态的独立预后指标。ＰＣＲ－ＳＳＣＰ分析表明，１５例ｐ５３基因表达阳性病例中４例于外显子５，６例于外显子７有该基因的突变（６７％），说明乳腺癌ｐ５３基因突变也多发生于其突变热点部位。
In this paper, 58 cases of breast cancer c-erbB-2 oncogene and p53 tumor suppressor gene mutations were studied by immunohistochemistry and PCR-SSCP technology. The results showed that the expression of c-erbB-2 was 38% in breast cancer and the expression of p53 The positive rate of c-erbB-2 was negatively correlated with histological grade (P <0.01) and lymph node metastasis (P <0.01), but not with intraductal papilloma (P <0.01). There was a positive correlation between the expression of p53 and lymph node metastasis (P <0.01), and the tumor size of ≥2 cm. The expressions of both genes were correlated with the malignant transformation of mammary epithelial cells and Lymph node metastasis is closely related, but no significant correlation between the two genes; therefore, each gene change can be independent of lymph node status independent prognostic indicators. PCR-SSCP analysis showed that in 15 cases of p53 gene expression positive cases, 4 cases of exon 5 and 6 cases of exon 7 mutations (67%), indicating that mutations in breast cancer p53 gene also occurred in Mutation hot spots.