论文部分内容阅读
本文报道了一种通过移植体外培养的表皮治疗白癜风的新方法。以前治疗色素脱失的方法很多,如吸力—发疱表皮移植、小块移植等,但这些方法只适于面积较小的色素脱失。治疗大面积的白癜风则需要大量带有色素的表皮,作者应用体外培养的含有黑素细胞的表皮进行移植获得良好效果。以1％利多卡因局麻后,取股内侧约4×1.5cm大皮片,放入含Hank平衡盐、胎牛血清和抗生素的Eagle基本培养液中。用0.25％胰蛋白酶,置37℃下,消化90分钟,使表皮和真皮分开,轻轻刮下粘附在真皮上的基底细胞和黑素细胞。用吸管强力吹打后得到细胞悬液。再用金属网过滤。然后离心两次(1200rpm),并重悬在Eagle培养液中。用血
This article reports a new method of treating vitiligo by transplanted in vitro cultured epidermis. In the past, there are many ways to treat depigmentation, such as suction-blister epidermis transplantation and patch transplantation, but these methods are only suitable for smaller area pigmentation. Treatment of large areas of vitiligo will require a large number of pigmented epidermis, the authors apply in vitro cultured melanocyte-containing epidermal grafts for good results. With 1% lidocaine local anesthesia, take the inside of a large piece of about 4 × 1.5cm, placed in Hank balanced salt, fetal bovine serum and antibiotics Eagle basic medium. With 0.25% trypsin, set at 37 ℃, digestion for 90 minutes, the epidermis and dermis, gently scraping off the adhesion of the basal cells and melanocytes on the dermis. After pipetted strongly, the cell suspension was obtained. Then use metal mesh filter. Then centrifuged twice (1200 rpm) and resuspended in Eagle’s medium. Use blood