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[目的]检测口腔黏膜鳞癌原发灶不同距离外科切缘中p53、CerbB-2、EGFR的表达情况。[方法]采用荧光定量RT-PCR技术检测9例口腔黏膜鳞癌患者术后病理切片阴性、距离切缘1.0cm和1.5cm处的21个标本中p53、CerbB-2、EGFR的mRNA表达水平。[结果]21个标本中,p53、CerbB-2、EGFR的mRNA相对表达量分别为0.71±1.46、4.94±7.76、0.78±1.19,其中CerbB-2的表达量显著高于p53、EGFR的表达(F=5.783,P<0.01)。距切缘距离1.5cm处的CerbB-2表达显著低于距切缘距离1.0cm处的表达(9.38±9.53vs.0.91±1.10,P=0.02),而p53、EGFR表达与手术切缘的距离无显著相关性(P>0.05)。p53、CerbB-2、EGFR表达均与肿瘤分期、患者的年龄无显著性相关(P>0.05)。[结论]临床上通过联合检测口腔鳞癌手术切缘中p53、CerbB-2及EGFR的表达,有助于更好地评估手术切缘的安全性。对于手术切缘组织中CerbB-2高表达的患者,手术切缘大于1.5cm可能更合理。
[Objective] To detect the expression of p53, CerbB-2 and EGFR in surgical margins of primary mucosal squamous cell carcinoma of oral mucosa at different distances. [Method] The mRNA expression levels of p53, CerbB-2 and EGFR in 21 specimens of oral squamous cell carcinoma of the mouth were negative. The mRNA expression of p53, CerbB-2 and EGFR was detected in 21 specimens at 1.0 cm and 1.5 cm from the cut edge. [Results] The relative expression levels of p53, CerbB-2 and EGFR in 21 specimens were 0.71 ± 1.46, 4.94 ± 7.76 and 0.78 ± 1.19, respectively. The expression of CerbB-2 was significantly higher than that of p53 and EGFR ( F = 5.783, P <0.01). The expression of CerbB-2 at a distance of 1.5 cm from the cut edge was significantly lower than that at the 1.0 cm distance from the cut edge (9.38 ± 9.53 vs.0.91 ± 1.10, P = 0.02) No significant correlation (P> 0.05). The expression of p53, CerbB-2 and EGFR had no significant correlation with tumor stage and patient’s age (P> 0.05). [Conclusion] The combined detection of p53, CerbB-2 and EGFR in the surgical margins of oral squamous cell carcinoma can help to evaluate the safety of surgical margins. For patients with high CerbB-2 expression in surgical margins, surgical margins greater than 1.5 cm may be more reasonable.