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目的:观察姜黄素对大鼠脑缺血-再灌注损伤的影响及其机制。方法:制作SD大鼠全脑缺血-再灌注损伤模型。实验设假手术组、模型组及姜黄素低、中、高剂量组,检测并比较各组大鼠脑组织损伤程度、白细胞浸润、神经元肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)及血脑屏障通透性随脑缺血-再灌注损伤时间表达变化的情况。结果:对应时间点,模型组及姜黄素组大鼠在脑损伤评分、白细胞数量、MMP-9表达、TNF-α表达及伊文思蓝含量均高于假手术组(P<0.05);姜黄素各剂量组脑损伤评分、白细胞数量、MMP-9表达、TNF-α表达及伊文思蓝含量显著低于相应模型组(P<0.05),以中剂量作用最佳。结论:姜黄素可减轻脑缺血病理损害,其机制可能与抑制神经元缺血-再灌注后白细胞的浸润、MMP-9、TNF-α表达及改善脑组织血脑屏障通透性有关。
Objective: To observe the effect of curcumin on cerebral ischemia-reperfusion injury in rats and its mechanism. Methods: The model of global cerebral ischemia - reperfusion injury in SD rats was made. The rats in the sham operation group, model group and curcumin low, middle and high dose groups were used to detect and compare the damage degree of brain tissue, the infiltration of leukocytes, the expression of tumor necrosis factor-α (TNF- α, matrix metalloproteinase-9 (MMP-9) and the permeability of the blood-brain barrier with the time of cerebral ischemia-reperfusion injury. Results: At the corresponding time points, the brain injury score, the number of white blood cells, the expression of MMP-9, the expression of TNF-α and the concentration of Evans blue in model group and curcumin group were significantly higher than those in sham operation group (P <0.05) The brain injury score, the number of white blood cells, the expression of MMP-9, the expression of TNF-α and the Evans blue content in each dose group were significantly lower than those in the corresponding model group (P <0.05). CONCLUSION: Curcumin can attenuate the pathological damage of cerebral ischemia. The mechanism may be related to the inhibition of the infiltration of leukocytes, the expression of MMP-9 and TNF-α and the permeability of the blood-brain barrier in brain tissue after ischemia-reperfusion injury.