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目的 :探讨反义寡核苷酸c fos对谷氨酸处理培养海马神经元的保护作用。方法 :应用MTT法 (四甲基偶氮唑盐微量酶反应比色法 )检测c fos反义寡核苷酸在不同浓度和时间对谷氨酸处理原代培养海马神经细胞的光密度值。结果 :c fos反义寡核苷酸在不同浓度和时间下对谷氨酸处理原代培养海马神经细胞光密度值均有增加。结论 :c fos反义寡核苷酸对谷氨酸毒性损伤的培养海马神经元有保护作用
Objective: To investigate the protective effect of antisense oligonucleotide c fos on glutamate-treated hippocampal neurons. Methods: MTT assay (MTT assay) was used to detect the optical density of c fos antisense oligodeoxynucleotides in primary culture of hippocampal neurons with different concentration and time. Results: The c fos antisense oligonucleotides showed an increase in optical density of primary cultured hippocampal neurons treated with glutamic acid at different concentrations and times. Conclusion: c fos antisense oligonucleotides have protective effects on cultured hippocampal neurons induced by glutamate toxicity