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目的:研究锰作用下PC12细胞的增殖抑制作用与凋亡相关的形态学、生化指标改变。方法:用200,400,600,800μmol/LMnCl2的培养液,分别作用对数生长期PC12细胞1,2,3,4d后,用MTT筛选锰的细胞毒性剂量;透射电镜观察细胞形态学变化;琼脂糖凝胶电泳检测MnCl2对PC12细胞基因组DNA的影响。结果:MTT实验显示200-800μmol/L MnCl2作用4天对PC12有显著的抑制作用,呈剂量和时间依赖趋势,600μmol/L MnCl2作用4d对PC12的抑制率可达50%以上。600μmol/L MnCl2作用4d电镜可见细胞凋亡,同样条件下细胞DNA碎片化。结论:PC12细胞在锰作用下发生增殖抑制,原因是锰诱导PC12细胞凋亡。
OBJECTIVE: To study the effects of manganese on the proliferation inhibition of PC12 cells and the changes of apoptosis-related morphological and biochemical indexes. Methods: The cytotoxic dose of manganese was screened by MTT using 200, 400, 600, 800μmol / L MnCl2 culture medium for 1, 2, 3 and 4 days respectively. The morphological changes of cells were observed by transmission electron microscope. The agarose gel electrophoresis The effect of MnCl2 on the genomic DNA of PC12 cells was examined. Results: MTT assay showed that PC12 was significantly inhibited by 200-800μmol / L MnCl2 for 4 days, and dose-dependent and time-dependent. The inhibition rate of PC12 by 600μmol / L MnCl2 for 4 days was more than 50%. Cell apoptosis was observed by electron microscopy with 600μmol / L MnCl2 for 4 days, and DNA fragmentation was also observed under the same conditions. Conclusion: Proliferation inhibition of PC12 cells under the action of manganese is due to manganese-induced apoptosis of PC12 cells.