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目的:探讨茶多酚对二乙基亚硝胺(DEN)诱发大鼠肝癌前病变的作用。方法:30只Wistar大鼠随机分为茶多酚组、DEN对照组和正常对照组,分别给予相应试药后除正常对照组外采用DEN和二乙基氨基芴诱发大鼠肝癌前病变,之后断颈处死大鼠取肝组织作γ-谷氨酰转肽酶(γ-GT)组化染色,并测定肝细胞浆中谷胱甘肽S-转移酶(GST)的活性和肝组织中GSTA1mR-NA的表达水平。结果:茶多酚组单位面积γ-GT阳性灶的总面积和个数均明显少于DEN对照组(P<0·01);茶多酚组的GST活性和GSTA1mRNA表达水平明显高于DEN对照组和正常对照组(P<0·01)。结论:茶多酚可通过调节GST活性达到预防和抑制DEN诱导的肝癌前病变的目的。
Objective: To investigate the effect of tea polyphenols on preneoplastic lesion induced by diethylnitrosamine (DEN) in rats. Methods: Thirty Wistar rats were randomly divided into two groups: the tea polyphenols group, the DEN control group and the normal control group. The rats were given DEN and diethylaminofluorene, respectively, except for the normal control group, The rats were sacrificed and their liver tissues were harvested for γ-glutamyl transpeptidase (γ-GT) staining. The activity of glutathione S-transferase (GST) in liver cytoplasm and the activity of GSTA1mR- NA expression level. Results: The total area and number of γ-GT positive foci in tea polyphenols group were significantly less than those in DEN control group (P <0.01). The GST activity and GSTA1 mRNA expression in tea polyphenols group were significantly higher than those in DEN control group Group and normal control group (P <0.01). Conclusion: Tea polyphenols can prevent and suppress DEN-induced precancerous lesions of liver cancer by regulating GST activity.