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采用清除二苯代苦味肼基(DPPH)自由基、清除[2,2′-连氨-(3-乙基苯并噻唑啉-6-磺酸)二氨盐](ABTS)自由基及铁离子还原/抗氧化能力(FRAP)测定法,对开封产的3种白色菊花(兼六香白、国华万胜及白玉带)不同溶剂提取物的体外抗氧化活性进行了评价,将所测定结果与水溶性维生素E(6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylic acid,Trolox)及阳性对照二丁基羟基甲苯(BHT)进行了比较。结果表明,3种菊花的不同溶剂提取物抗氧化活性不同。同一种菊花的甲醇提取物具有很好清除DPPH自由基和还原铁离子的能力,而石油醚提取物几乎无活性。菊花的3个品种中,兼六香白和国华万胜的抗氧化活性较好,其活性远远超过白玉带的抗氧化活性。9个提取物中,兼六香白的甲醇提取物总的抗氧化活性最好。它对DPPH自由基的清除能力(IC50值为20.49 mg/L)比BHT(IC50值为18.92 mg/L)作用略低;其还原Fe3+的能力(FRAP值为731.73±1.77μmol TE/g)比BHT(1 581.68±97.41μmol TE/g)作用低1/2。3种方法测定结果基本一致,其中以DPPH法和FRAP法相关性最好(R=0.982 0)。
Use of DPPH free radicals, removal of [2,2’-paraxine-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] (ABTS) radicals and iron The ionic reduction/antioxidant capacity (FRAP) assay evaluated the in vitro antioxidant activity of different solvent extracts of three kinds of white chrysanthemum (Kaixiangbai, Guohua Wansheng and Baiyu) produced in Kaifeng. The results were compared with water-soluble vitamin E (6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylic acid, Trolox) and the positive control dibutylhydroxytoluene (BHT). The results showed that the different solvent extracts of the three chrysanthemums have different antioxidant activities. Methanol extracts of the same chrysanthemum have a good ability to scavenge DPPH radicals and reduce iron ions, while petroleum ether extracts are almost inactive. Among the three varieties of chrysanthemum, the antioxidant activity of both Liuxiangbai and Guohua Wansheng is better, and its activity far exceeds the antioxidative activity of Baiyudai. Among the nine extracts, the total antioxidant activity of the hexamethylene white methanol extract was the best. It has slightly lower DPPH free radical scavenging capacity (IC50 value 20.49 mg/L) than BHT (IC50 value 18.92 mg/L); its ability to reduce Fe3+ (FRAP value is 731.73±1.77 μmol TE/g) The effect of BHT (1 581.68±97.41 μmol TE/g) was lower by 1/2. The results of the three methods were basically the same, and the DPPH method and FRAP method had the best correlation (R=0.982 0).