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本文对有机磷抗性和感性棉蚜Aphis gossypii三个种群抗性生化机制进行了讲究。首先用解毒酶的抑制剂测定药剂的解毒途径。进一步测定乙酰胆碱酯酶活力及其敏感性和多功能氧化酶、谷胱甘肽s-转移酶、α-乙酸萘酯酶和α-乙酸萘酯羧酸酯酶等解毒酶的活力。结果表明,体内条件下,多功能氧化酶与抗性有关,但在离体条件下,在棉蚜匀浆液中有内源抑制剂存在。α-乙酸萘酯酶和α-乙酸萘酯羧酸酯酶活力的增加,乙酰胆碱酯酶对杀虫剂敏感性的降低也是造成棉蚜对有机磷产生抗性的原因。
In this paper, the biochemical mechanisms of resistance to three organism phosphorus resistance and susceptible cotton aphids Aphis gossypii were investigated. First use detoxification inhibitors to determine the agent’s detoxification pathway. The activity of acetylcholinesterase and its sensitivity and the activities of multi-functional oxidase, glutathione s-transferase, α-naphthyl acetate esterase and α-naphthyl acetate carboxylesterase were further determined. The results showed that under the in vivo conditions, the multifunctional oxidase is related to resistance, but in vitro, endogenous inhibitors exist in the cotton aphid homogenate. The increase of α-naphthyl acetate esterase and α-naphthyl acetate carboxylesterase activity, the decrease of sensitivity of insecticides to acetylcholinesterase is also the reason of the resistance of cotton aphid to organic phosphorus.