论文部分内容阅读
目的：观察脑室注射神经生长因子（NGF）对大鼠学习记忆以及自发行为的影响。方法：以18月龄大鼠为材料，侧脑室多次注射NGF（累计剂量55μg·只(-1)）．同龄对照组注射等体积人工脑积液（ACSF）。采用开场行为和Y-迷宫实验检测了大鼠自发活动和学习记忆能力的改变。行为实验后分离主要脑区（皮层、海马、小脑、间脑）的突触体，使用3H－Leu为标记物检测了离体突触体总蛋白的合成量。结果：NGF能提高大鼠的自发活动和学习记忆能力，并能提高与学习、运动相关脑区的突触体蛋白质合成。结论：提示NGF对大鼠自发活动和学习记忆的影响与学习、运动相关脑区的突触部位蛋白质合成能力提高有关。
Objective: To observe the effects of intraventricular injection of nerve growth factor (NGF) on learning and memory and spontaneous behavior in rats. Methods: 18-month-old rats were used as the material. NGF was injected into the lateral ventricle repeatedly (total dose 55μg · only (-1)). The same age control group was injected with equal volume of artificial brain effusion (ACSF). The opening behavior and Y-maze test were used to detect the changes of spontaneous activity and learning and memory ability in rats. After behavioral experiments, synaptosomes in major brain regions (cortex, hippocampus, cerebellum, and diencephalon) were isolated and 3H-Leu was used as a marker to detect the total amount of synaptosomal protein synthesis. Results: NGF increased spontaneous activity, learning and memory in rats, and increased synaptophysin synthesis in brain regions related to learning and exercise. CONCLUSION: The effect of NGF on spontaneous activity and learning and memory in rats is related to the increased ability of protein synthesis in the synapses of learning and motor related brain regions.