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目的:建立气相色谱法(GC)测定不同产地牛蒡子脂肪油中亚油酸、油酸、棕榈酸含量的方法。方法:采用PEG-20M弹性石英毛细管柱(0.5μm×0.32 mm×30 m);气化室温度280℃,FID检测器,温度300℃,柱温初温165℃,保持3min,以3.5℃.min-1升至205℃,然后以35℃.min-1升至230℃,保持22 min。载气氮气(99.99%)。以石油醚为提取溶媒,提取10批不同产地牛蒡子药材生品及5批不同产地炮制品中的脂肪油,经甲酯化后,以十七烷酸甲酯为内标物,进行测定比较。结果:亚油酸在0.122 3～1.958 1μg线性关系良好(r=0.999 8);油酸在0.039 2～0.626 4μg线性关系良好(r=0.999 8);棕榈酸在0.037 2～0.596 4μg线性关系良好(r=0.999 8)。各产地牛蒡子脂肪油中均以亚油酸含量为最高,炮制前后对脂肪酸含量的影响较大。结论:方法准确、可靠、重复性好,可用于牛蒡子脂肪油中亚油酸、油酸、棕榈酸的质量控制。
Objective: To establish a method for the determination of linoleic acid, oleic acid and palmitic acid in Fatty Oil of Arctium lappa L. from different areas by gas chromatography (GC). METHODS: PEG-20M elastic quartz capillary column (0.5 μm × 0.32 mm × 30 m) was used. The gasification chamber temperature was 280 ℃ and the FID detector was used. The temperature was set at 300 ℃. min-1 to 205 ° C and then to 230 ° C at 35 ° C. min-1 for 22 min. Carrier gas nitrogen (99.99%). Petroleum ether as the extraction solvent, extracted from 10 different origin Arctii raw material and 5 batches of different origin of fatty oil products, after methyl esterification, methyl palmitate as an internal standard, were measured and compared . Results: The linear relationship of linoleic acid between 0.122 3 ~ 1.958 1μg was good (r = 0.999 8), oleic acid was good at 0.039 2 ~ 0.626 4μg (r = 0.999 8), palmitic acid was good at 0.037 2 ~ 0.596 4μg (r = 0.999 8). Burdock fatty oil in the origin of linoleic acid were the highest, before and after processing on the fatty acid content greater impact. Conclusion: The method is accurate, reliable and reproducible. It can be used for the quality control of linoleic acid, oleic acid and palmitic acid in the fatty oil of Arctium lappa L.