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目的：研究５ＨＴ对ＳＴＡ２血小板聚集和释放反应的影响及可能的分子机制．方法：以透光法，介质中ＡＴＰ含量及荧光图像法评价血小板变形，聚集反应和［Ｃａ２］ｉ水平．结果：（１）５ＨＴ预处理可消除ＳＴＡ２的血小板变形，ＳＴＡ２０３μｍｏｌ·Ｌ－１的聚集增强，ｌ－３μｍｏｌ·Ｌ－１的聚集不变，释放反应抑制．（２）５ＨＴ预处理增加ＳＴＡ２０３μｍｏｌ·Ｌ－１的［Ｃａ２＋］ｉ，降低３μｍｏｌ·Ｌ－１的［Ｃａ２＋］ｉ降低．（３）延长加入５ＨＴ和ＳＴＡ２的间隔，ＳＴＡ２０３μｍｏｌ·Ｌ－１的聚集增强，３μｍｏｌ·Ｌ－１的聚集不变，释放反应抑制．结论：５ＨＴ对ＳＴＡ２介导的聚集和释放反应有双重影响．对ＳＴＡ２［Ｃａ２＋］ｉ的调节可能是上述反应的分子机制．
Objective: To investigate the effect of 5HT on STA2 platelet aggregation and release and its possible molecular mechanism. Methods: Platelet deformability, aggregation and [Ca2] i levels were evaluated by light transmission, ATP content in media and fluorescence imaging. Results: (1) 5HT pretreatment can eliminate STA2 platelet deformation, STA20 3μmol·L-1 aggregation enhanced, l-3μmol·L-1 aggregation unchanged, the release of inhibition. (2) 5HT preconditioning increased [Ca2 +] i in STA203μmol·L-1 and decreased [Ca2 +] i in 3μmol·L-1. (3) The interval of adding 5HT and STA2 was prolonged, the aggregation of STA203μmol·L-1 was enhanced, the aggregation of 3μmol·L-1 was unchanged, and the release inhibition was inhibited. Conclusion: 5 HT STA2-mediated aggregation and release of a double reaction. The regulation of STA2 [Ca2 +] i may be the molecular mechanism of the above reaction.