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目的:观察六月青多糖(LYQP)对鸭乙型肝炎所致肝损伤的保护作用,为研究和开发新的抗乙型肝炎病毒(HBV)药物提供实验依据。方法:采用鸭乙型肝炎病毒(DHBV)诱导广西麻鸭乙型肝炎病毒肝损伤模型。分别检测给药前(T0)、给药7 d(T7),14 d(T14)及停药后3 d(P3)血清中丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)的活性,同时采用酶联免疫吸附(ELISA)法测定上清液鸭乙型肝炎病毒表面抗原(DHBsAg)和e抗原(DHBeAg)的滴度,HE染色观察肝损伤程度。结果:与模型组相比,各给药组鸭血清ALT,AST的活性及血清DHBsAg,DHBeAg的滴度显著降低(P<0.05或P<0.01);停药3 d后,LYQP高、中剂量组血清ALT,AST活性及血清DHBsAg,DHBeAg的滴度均无反跳现象。HE染色结果显示LYQP可显著减轻鸭肝组织病理损伤程度。结论:LYQP对DHBV所致肝损伤具有保护作用。
Objective: To observe the protective effect of LYQP on liver injury induced by Duck Hepatitis B, and to provide experimental basis for the research and development of new anti-Hepatitis B virus (HBV) drugs. Methods: Hepatitis B virus (HBV) induced liver injury in Guangxi ducklings was induced by duck hepatitis B virus (DHBV). Serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured before treatment (T0), 7 d after administration (T7), 14 d (T14) and 3 d The titers of DHBsAg and DHBeAg in the supernatant were determined by enzyme-linked immunosorbent assay (ELISA). The degree of hepatic injury was observed by HE staining. Results: Compared with the model group, the ALT and AST activities and the titer of serum DHBsAg and DHBeAg in each group were significantly decreased (P <0.05 or P <0.01). After 3 days’ withdrawal, the LYQP high and medium dose Serum ALT, AST activity and serum DHBsAg, DHBeAg titer no rebound phenomena. HE staining showed that LYQP could significantly reduce the degree of pathological damage in duck liver tissue. Conclusion: LYQP has a protective effect on liver injury induced by DHBV.