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目的 观察苯乙酸 (PA)和二甲基甲酰胺 (DMF)诱导分化胶质瘤细胞C6 过程中同源盒(Hox)基因表达的变化。方法 用逆转录 聚合酶链反应 (RT PCR)及图像分析法 ,检测PA、DMF对胶质瘤细胞C6 诱导分化过程中Hox基因组P1、P2、P3及特异Hox基因的表达。Hox基因(组 )表达水平用基因 (组 ) / β 肌动蛋白 (β actin)灰度比值表示。 结果 Hox基因组P2在胶质瘤细胞C6 中表达明显低于P1、P3组 [0 .682 5± 0 .0 987<0 .881 7± 0 .0 73 1 ,0 .860 8± 0 .0 881 ,(P<0 .0 0 1 ) ]。应用PA后 ,P2组HoxB2基因表达明显上调 [0 .776 3± 0 .1 2 4 1 >0 .483 9± 0 .1 34 3 ,(P <0 .0 0 1 ) ] ;应用DMF后 ,HoxB2基因表达无显著变化 ,差异无显著性 (P >0 .0 5)。HoxB4基因在应用PA和DMF前后均未见表达。结论 PA对HoxB2基因mRNA水平的表达有明显的上调作用 ,DMF对Hox基因表达无显著影响
OBJECTIVE: To observe the changes of Hox gene expression in C6 glioma cells induced by phenylacetate (PA) and dimethylformamide (DMF). Methods Reverse transcription polymerase chain reaction (RT PCR) and image analysis were used to detect the expression of Hox P1, P2, P3 and Hox genes in glioma C6 cells induced by PA and DMF. The expression level of Hox gene (group) is expressed as the gray ratio of the gene / β actin. Results The expression of Hox genomic P2 in C6 glioma cells was significantly lower than that in P1 and P3 groups [0.682 5 ± 0.987 <0.881 7 ± 0.073.1,0.860 8 ± 0.0881 , (P <0 .0 0 1)]. After application of PA, the expression of HoxB2 in P2 group was significantly up-regulated [0 .776 3 ± 0 .1 2 4 1> 0.483 9 ± 0. 1 34 3, (P <0. 001)]; after DMF was applied, There was no significant difference in HoxB2 gene expression (P> 0.05). HoxB4 gene was not expressed before and after the application of PA and DMF. Conclusion PA can significantly up-regulate the expression of HoxB2 mRNA, while DMF has no significant effect on Hox gene expression