作者通过一系列实验,对4种缝线(丝线、单丝尼龙线、多丝尼龙线、多聚二醇酸线)缝合腹部感染切口的效果进行比较。材料和方法 1.带菌数——将金黄色葡萄球菌菌落接种至10ml 肉汤中,取3ml 移至一试管(试管 A)中,内置一段50 mm 长无菌缝线,在37℃下置60分钟,然后无菌地取出缝线,放入3 ml 无菌肉汤(试管 B)中,用旋转搅拌器搅拌3分钟,以洗掉缝线表面的细菌,再取出缝线。假设未放缝线前每 ml 肉汤内含细菌数为C,试管 A 和试管 B 肉汤每 ml 含细菌数分别为 A 和 B,则每段缝线内带菌数 S 等于 C-(A+B)。 Through a series of experiments, the authors compared the effects of suturing abdomen incisions with four sutures (silk, monofilament nylon thread, multifilament nylon thread, polyglycolic acid thread). Materials and methods 1. Number of bacteria-inoculated Staphylococcus aureus colonies in 10ml broth, take 3ml moved to a test tube (tube A), built-in a 50mm long sterile suture, set at 37 ℃ 60 Min, then aseptically remove the suture, place it in 3 ml of sterile broth (tube B) and stir for 3 minutes with a rotary stirrer to wash off the bacteria on the surface of the suture and remove the suture. Assuming that the number of bacteria contained per ml of broth prior to suturing is C and that the number of bacteria per ml in tubes A and B is A and B, respectively, the number of inocula in each suture is equal to C- (A + B).