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利用Ｎｏｒｔｈｅｒｎ印迹杂交及逆转录聚合酶链式反应（ＲＴ－ＰＣＲ）技术研究了几种传代培养细胞和成人肝组织及人胚胎肝组织细胞色素Ｐ４５０１Ａ１（１Ａ１）基础表达水平并观察了几种化学诱导剂对人羊膜细胞ＦＬ系和原代胚肝细胞该基因ｍＲＮＡ水平的诱导．结果显示：３－甲基胆蒽，苯巴比妥，β－萘黄酮在不同程度上能高诱导ＦＬ细胞１Ａ１ｍＲＮＡ表达．ＲＴ－ＰＣＲ结果显示ＣＨＬ细胞及Ｖ７９细胞存在基础表达，但Ｎｏｒｔｈｅｒｎ杂交结果提示其表达量极低．成人肝脏中１Ａ１亦存在极低的表达水平，而人胚胎肝脏不能用高灵敏度的ＲＴ－ＰＣＲ检测到痕迹表达，提示胚胎发育时期１Ａ１基因处于完全关闭状态．
The basic expression level of cytochrome P4501A1 (1A1) in several subcultured cells, adult liver tissues and human embryos was studied by Northern blotting and reverse transcriptase polymerase chain reaction (RT-PCR), and several chemical induction Agent on the induction of mRNA levels of this gene in human amniotic FL cells and primary embryonic hepatocytes. The results showed that 3-methylcholanthrene, phenobarbital and β-naphthoflavone could highly induce 1A1mRNA expression in FL cells. The results of RT-PCR showed that the basic expression of CHL cells and V79 cells existed, but the results of Northern blot showed that the expression level was extremely low. 1A1 in adult liver also had a very low expression level, while human embryonic liver could not detect the trace expression by high-sensitivity RT-PCR, suggesting that the 1A1 gene in embryo development was in a completely closed state.