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给大鼠一次腹腔注射1.5％粗制木瓜酶溶液(按150mg/kg体重计量),可明显引起大鼠软骨基质可复性损伤。注射后6h,实验组大鼠软骨硫酸软骨素(CH·S)含量便开始下降,至24h仅为对照组含量的一半,同时软骨CH·S有机硫含量明显减少。实验组大鼠尿液糖胺多糖(GAG)排泄量子注射后12h开始升高,24h达到最高峰,为对照组排泄量的3倍,GAG排泄量的增加主要成分为CH·S。上述代谢损伤至72h可完全恢复正常。实验证明,木瓜酶对软骨CH·S分子量无明显影响。
Rats were given an intraperitoneal injection of a 1.5% crude papain enzyme solution (measured at 150 mg/kg body weight), which markedly induced rat cartilage matrix remodeling injury. Six hours after injection, the content of cartilage chondroitin sulfate (CH·S) in the experimental group began to decrease, and it was only half of the content in the control group at 24 hours. At the same time, the CH and S organic sulfur content in the cartilage decreased significantly. The urinary glycosaminoglycan (GAG) excretion in the experimental group began to increase 12 h after the quantum injection, reached the highest peak at 24 h, and was 3 times the excretion of the control group. The main component of GAG excretion increased was CH·S. The above metabolic damage can be completely restored to normal after 72 hours. Experiments show that papain has no obvious effect on the molecular weight of CH.