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应用 ABC 免疫组化技术,研究了海兔5-羟色胺免疫反应阳性(5-HT-IR)纤维在侧节感觉神经元簇(PSC)中的分布及其与侧节感觉神经元(PSN)的关系。可见 PSN 胞体和轴突的近侧段被含有大量膨体的5-HT-IR 纤维所包绕。光镜下测量4个视野区的5-HT-IR,膨体平均短径为1.1±0.6μm,分布范围为0.2～3.8μm。膨体大小的频数分布呈正偏态,79%的膨体短径分布于0.2～1.4μm 之间。5-HT-IR 纤维至少存在4种类型。少数5-HT-IR 纤维可有分支发出,发出点通常是在膨体上,从膨体发出2～4条细分支,每条分支上又可见数个子膨体存在。发出分支的膨体平均短径为1.70±0.5μm,分布范围在1.0～2.4μm,某些小的膨体似与 PSN 胞体表面形成接触,但现有结果尚不足以说明5-HT-IR 纤维与 PSN 之间存在着突触样联系,因为 PSN 细胞胞体表面有胶质细胞形成的胶质鞘包绕,这一结构或许阻碍5-HT-IR 纤维与 PSN 形成真正的突触联系。
The distribution of serotonergic immunoreactive (5-HT-IR) fibers in lateral serous sensory neuron clusters (PSC) and their relationship with lateral sensory neurons (PSNs) were studied by ABC immunohistochemistry relationship. It can be seen that the proximal segments of PSN soma and axons are surrounded by 5-HT-IR fibers containing a large amount of bulge. The 5-HT-IR of four visual fields were measured under light microscope. The average short diameter of bulge was 1.1 ± 0.6μm and the distribution range was 0.2 ~ 3.8μm. The frequency distribution of the bulge size is positively skewed, and 79% of the bulky short diameters are distributed between 0.2 and 1.4 μm. There are at least 4 types of 5-HT-IR fibers. A small number of 5-HT-IR fibers may have branches issued, the point of issue is usually in the bulge, issued from the bulge 2 to 4 fine branches, each branch can be seen on the number of bulges exist. The average diameter of the emanated branches was 1.70 ± 0.5 μm and the distribution range was 1.0 ~ 2.4 μm. Some small bulges appeared to be in contact with the surface of PSN somatic cells. However, the available results are not enough to show that 5-HT-IR fibers and There is a synaptic connection between PSNs because the PSN cell surface is surrounded by glia-forming glia. This structure may prevent the 5-HT-IR fibers from forming a true synaptic connection with PSN.