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利用转抗白叶枯病基因Xa2 1的水稻材料 ,通过TAIL PCR方法扩增T DNA整合的侧翼序列。从中筛选属于水稻基因组DNA的T DNA整合的侧翼序列作为探针 ,将外源基因整合位点定位到窄叶青 /京系 17DH群体构建的水稻分子连锁图谱上。共获得属于水稻基因组DNA的T DNA侧翼序列 2 2个 ,其中的 19个序列在定位群体的两个亲本之间显示RFLP多态性 ,分别定位在水稻基因组的第 3,4 ,5 ,7,9,10 ,11和 12染色体上。带有转基因Xa2 1的T DNA整合的定位为研究外源基因在不同染色体位点的位置效应和稳定遗传打下基础。
The flanking sequences of T DNA integration were amplified by TAIL PCR using rice material transformed with the bacterial blight resistance gene Xa21. The flanking sequences of T DNA that belonged to rice genomic DNA were screened out as probes and the foreign gene integration sites were mapped to the molecular linkage map of rice constructed by Z. japonica / Jing 17DH population. A total of 22 T DNA flanking sequences were obtained from rice genomic DNA. Among them, 19 sequences showed RFLP polymorphisms between two parents of the loci, and were mapped to the 3, 4, 5, 7, 9, 10, 11 and 12 chromosomes. The localization of T DNA integration with transgene Xa2 1 lays the foundation for studying the positional effect and stable inheritance of exogenous genes at different chromosomal loci.