目的采用抗ＣＤ２８，ＣＤ８０，ＣＤ２及ＣＤ５８分别刺激健康人ＰＢＬ后作用肝癌细胞，对作用前后ＰＢＬ的表型变化及ＴＣＲＶβ基因亚家族的表达水平进行探讨．方法用ＦＡＣＳ分析作用肝癌细胞前后ＰＢＬ表型变化，并用ＲＴＰＣＲＳｏｕｔｈｅｒｎ印迹分析其ＴＣＲＶβ１２０的表达水平及特征．结果健康人ＰＢＬ作用肝癌细胞后ＣＤ３和ＣＤ８分子表达比作用前明显增高，而ＣＤ４分子无显著变化．健康人ＰＢＬ分别加ＩＬ２，ＰＨＡ，抗ＣＤ３和ＣＤ３＋ＣＤ２８，ＣＤ２８＋ＣＤ８０，ＣＤ２＋ＣＤ５８作用肝癌细胞（ＢＥＬ７４０２）前表达水平平均约５％，作用ＢＥＬ７４０２后表达水平约１３％～２５％，其特征为Ｖβ７增高．结论在癌抗原的参与下，ｍＡｂ共刺激的Ｔ细胞活化，ＴＣＲ接受ＡＰＣ呈递的相应抗原的刺激，具有该ＴＣＲ的淋巴细胞迅速增殖而成为针对抗原的Ｔ细胞克隆，发挥其识别和杀伤癌细胞的作用 Objective To use anti-CD28, CD80, CD2 and CD58 to stimulate healthy human PBL to act on hepatocellular carcinoma cells, and investigate the phenotypic changes of PBL and the expression levels of TCRVβ subfamily before and after treatment. Methods The phenotypes of PBL before and after hepatocellular carcinoma cells were analyzed by FACS. The expression and characteristics of TCRVβ120 were analyzed by RTPCRSouthern blot. RESULTS: The expression of CD3 and CD8 molecules in hepatoma cells after PBL treatment was significantly higher than that before exposure, but there was no significant change in CD4 molecules. The expression levels of IL-2, PHA, anti-CD3 and CD3+CD28, CD28+CD80, and CD2+CD58 in healthy human PBLs before hepatocarcinoma cells (BEL-7402) were approximately 5%, and those in BEL-7402 cells were approximately 13%-25%. Features increased Vβ7. Conclusion With the participation of cancer antigens, the T cells stimulated by mAbs are activated and TCR is stimulated by the corresponding antigens presented by APC. The lymphocytes with this TCR rapidly proliferate and become T cell clones against the antigen, exerting their functions of recognizing and killing cancer cells. The role of