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通过SELEX技术已经筛选到了多种HIV-1病毒相关蛋白,如:逆转录酶、Rev和Tat蛋白、核壳蛋白等的核酸适配子,然而以HIV-1整合酶为靶点的DNA或RNA适配子却鲜有报道.在结合缓冲液中以10mmol·L-1的Mg2+作为辅助因子,加入100mmol·L-1KCl筛选到了三个具有相似结构和Kd的适配子IN1,IN2和IN3.亲和力最高的适配子IN1的Kd为145nmol·L-1,保守区富含鸟嘌呤碱基(G),缺失任何一个茎环结构会使亲和力减弱.缺失右端固定序列的IN1-L的Kd为283nmol·L-1,虽然不能形成四链结构但可以被G-四链T40214竞争结合,可能与T40214具有相同的结合位点.
A variety of HIV-1 virus-related proteins have been screened by SELEX technology, such as reverse transcriptase, Rev and Tat proteins, nucleocapsid protein aptamers, whereas DNA or RNA targeting HIV-1 integrase Aptamers were seldom reported.In the binding buffer, 10 mmol·L-1 Mg2 + was added as a cofactor, and three adapters IN1, IN2 and IN3 with similar structure and Kd were selected by adding 100 mmol·L-1 KCl. The highest affinity adapter IN1 Kd 145nmol·L-1, the conserved region is rich in guanine bases (G), the absence of any one of the stem-loop structure will make the affinity weakened.Deleted the right fixed sequence IN1-L Kd 283nmol·L-1, although it can not form a four-chain structure but can be competitively bound by G-four-chain T40214, may have the same binding site as T40214.