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Objective:To investigate the potential antioxidant effects of Phyllanthus niruri(P.niruri.Euphorhiaceae)tea on healthy subjects.Methods:Five non-smoking,male healthy volunteers,20 to 31 years old.were enrolled.Kach subject was treated twice,following a randomized crossover fashion regarding the ingestion of P.niruri infusion(5 g/750 mL)(tea group)or 750 mL of water(control group).Fasting venous blood samples were collected prior to and at 1.2 and 4 h after infusion drinking.Samples were tested for plasmatic gallic acid and ascorbic acid levels,erythrocytic catalase and superoxide dismutase activities,and intracellular DCFH fluorescence in granulocytes,monocytes and lymphocytes.Results:Catalase and superoxide dismutase activities were not altered by tea ingestion.Plasma levels of gallic acid were significantly increased at1,2 and 4 h after P.niruri ingestion and plasma ascorbic acid at 1 h after P.niruri ingestion.Conclusions:Ingestion of P.niruri tea is associated with a slight increase in antioxidant markers in human blood(ascorbic acid and gallic acid),which may contribute to its pharmacological effects.
Objective: To investigate the potential antioxidant effects of Phyllanthus niruri (P. niruri. Euphorhiaceae) tea on healthy subjects. Methods: Five non-smoking, male healthy volunteers, 20 to 31 years old. Were enrolled. Kach subject was treated twice, following a randomized crossover fashion regarding the ingestion of P. niruri infusion (5 g / 750 mL) (tea group) or 750 mL of water (control group) .Fasting venous blood samples were collected prior to and at 1.2 and 4 h after infusion drinking . Samples were tested for plasmatic gallic acid and ascorbic acid levels, erythrocytic catalase and superoxide dismutase activities, and intracellular DCFH fluorescence in granulocytes, monocytes and lymphocytes. Results: Catalase and superoxide dismutase activities were not altered by tea ingestion. Plasma levels of gallic acid were significantly increased at 1,2 and 4 h after P. niruri ingestion and plasma ascorbic acid at 1 h after P. niruri ingestion. Confc: Ingestion of P. niruri tea is associated with a slight increas e in antioxidant markers in human blood (ascorbic acid and gallic acid), which may contribute to its pharmacological effects.